Journal: The EMBO Journal

Article Title: Somatostatin triggers local cAMP and Ca 2+ signaling in primary cilia to modulate pancreatic β-cell function

doi: 10.1038/s44318-025-00383-7

Figure Lengend Snippet: ( A ) Confocal microscopy image of a primary cilium (acetylated tubulin; magenta) in a human islet that expresses SSTR5 (green). ( B ) Confocal microscopy image of primary cilia (acetylated tubulin; magenta) in a mouse islet that expresses SSTR5 (green). ( C ) Confocal microscopy image of a primary cilium (acetylated tubulin; magenta) in a MIN6 pseudoislet that lack expression of SSTR5 (green). ( D ) An illustration shows the membrane localization of SSTRs, with IC3 loop indicated by the purple box. Sequences of mSSTR2, mSSTR3 and mSSTR5. Conserved motif RxRxxR is highlighted. ( E ) Sequences of SSTR5 and SSTR3 showing the evolutionary conservation of a stretch of amino acids in purple.

Article Snippet: The following primary antibodies were used in the study; acetylated tubulin (T745, host: mouse, 1:500; Sigma-Aldrich), Acetyl-α-Tubulin (K40) (5335, host: rabbit, 1:500; BioNordika), SSTR3 (E-AB-16077, host: rabbit, 1:200; Elabscience), Arl13b (ab136648, host: mouse, 1:300; Abcam), SST (A0566, host: rabbit, 1:500, Dako), Insulin (A0564, host: guinea pig, 1:500; Dako), SSTR5 (66772-1-Ig, host: mouse, 1:200, Proteintech), SSTR2 (ab134152, host: rabbit, 1:200, Abcam), Pericentrin (ab4448, Abcam, host: rabbit, 1:200), GABBR1 (AGB001AN102, Alomone labs, host: rabbit, 1:200), GLP-1R (Mab 7F38-s, Developmental Studies Hybridoma Bank, host: mouse, 1:10) and GFP (AB16901, Abcam, host: chicken, 1:500).

Techniques: Confocal Microscopy, Expressing, Membrane

Journal: The EMBO Journal

Article Title: Somatostatin triggers local cAMP and Ca 2+ signaling in primary cilia to modulate pancreatic β-cell function

doi: 10.1038/s44318-025-00383-7

Figure Lengend Snippet: Reagents and tools table

Article Snippet: The following primary antibodies were used in the study; acetylated tubulin (T745, host: mouse, 1:500; Sigma-Aldrich), Acetyl-α-Tubulin (K40) (5335, host: rabbit, 1:500; BioNordika), SSTR3 (E-AB-16077, host: rabbit, 1:200; Elabscience), Arl13b (ab136648, host: mouse, 1:300; Abcam), SST (A0566, host: rabbit, 1:500, Dako), Insulin (A0564, host: guinea pig, 1:500; Dako), SSTR5 (66772-1-Ig, host: mouse, 1:200, Proteintech), SSTR2 (ab134152, host: rabbit, 1:200, Abcam), Pericentrin (ab4448, Abcam, host: rabbit, 1:200), GABBR1 (AGB001AN102, Alomone labs, host: rabbit, 1:200), GLP-1R (Mab 7F38-s, Developmental Studies Hybridoma Bank, host: mouse, 1:10) and GFP (AB16901, Abcam, host: chicken, 1:500).

Techniques: Recombinant, Plasmid Preparation, Sequencing, Virus, Cloning, Modification, Software, Microscopy, Real-time Polymerase Chain Reaction, SYBR Green Assay, Ligation

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 mRNA expression in CRC patients and in histologically normal colorectal mucosa from the same patients (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal expression of SST and SST1-5 mRNA in colorectal cancer samples (CRC) and non-tumor samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Incidence of somatostatin (SST) and SST receptor (SST1-5) expression in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Detection of IHC expression pattern of SST receptors (SST1-5) in colorectal cancer (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Expressing, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of the quantitative SST and SST1-5 immunoexpression in colorectal cancer (CRC), lymph node metastasis (LNM), and control mucosa (C) (A%).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between reciprocal immunoexpression of SST and SST1-5 in colorectal cancer samples (CRC), lymph node metastasis (LNM), and control colorectal mucosa (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 expression in relation to different IHC patterns of cellular localization.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and or SST1-5 (mRNA and peptide) in colorectal cancer (CRC) and control mucosa samples (C).

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST1-5 immunoexpression in colorectal cancer (CRC) and control samples (C) in relation to different anatomical sites of the large intestine.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Control

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 mRNA expression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous subtypes of CRC.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison, Expressing

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Comparison of SST and SST1-5 immunoexpression in colorectal cancer (CRC) in relation to mucinous vs. nonmucinous CRC subtypes.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Comparison

Journal: Cancers

Article Title: Differentially Expressed Somatostatin (SST) and Its Receptors (SST1-5) in Sporadic Colorectal Cancer and Normal Colorectal Mucosa

doi: 10.3390/cancers16213584

Figure Lengend Snippet: Values of Spearman’s coefficient for correlation between SST and SST1-5 mRNA in colorectal cancer (CRC), control colorectal mucosa (C), age of the patients, and basic laboratory tests.

Article Snippet: Rabbit polyclonal antibodies specific for SST1 (orb229340, at 1:500 dilution), SST2 (orb221903, at 1:500 dilution), SST3 (orb11423, at 1:50 dilution) (all Abs from Biorbyt Ltd., Cambridge, CB5 8LA, UK), SST4 (DF2779, at 1:200 dilution) (Affinity Biosciences (Middlesex, UK), www.affbiotech.com ), SST5 (orb11424, at 1:200 dilution) (Biorbyt Ltd.), and Ki-67 antigen (clone MIB-1) (Dako Denmark A/S, Glostrup, Denmark, ready to use) were used.

Techniques: Control